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epha3 py779  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc epha3 py779
    Epha3 Py779, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/epha3+py779/Phospho-EphA3+(Tyr779)+Rabbit+mAb/10__1016_slash_j__jbc__2021__100876-303-87-89
    Average 93 stars, based on 9 article reviews
    epha3 py779 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain
    Article Snippet: Lysates, pull-downs and immunoprecipitations were run on Bolt 4-12% Bis-Tris Plus gels (Invitrogen, NW04125). .. After semi-dry transfer, the immobilon membranes were blocked with 5% bovine serum albumin in 0.1% Tween-20 in TBS (150 mM NaCl, 50 mM TrisHCl pH 7.5) for 1 hour and then incubated overnight at 4°C in blocking buffer containing primary antibodies recognizing EphA4 (BD Biosciences, 610471; 1:1,000; Figs. 1D, 2A-C, 4B and 7A), an affinitypurified rabbit polyclonal EphA4 antibody generated using a peptide corresponding the the 11 C- terminal amino acids of human and mouse EphA4 (76) and used at 1 g/ml in Figs. 2D and 4A); EphA3 pY779 (Cell Signaling Technology, 8862; 1:1,000 dilution), which also recognizes EphA4 pY779; β-tubulin (Cell Signaling Technology, 2128; 1:25,000 dilution); the FLAG tag (Sigma, F1804; 1:1,000 dilution) and an HRP conjugated antibody recognizing phosphotyrosine (Cell Signaling Technology, 5465; 1:2,000). .. The Strep tag was detected by incubating the blots overnight with Strep-Tactin-HRP conjugate (IBA GmbH, 2-1502-001; 1:1,000 dilution).

    Article Title: A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain
    Article Snippet: Lysates, pull-downs, and immunoprecipitations were run on Bolt 4 to 12% Bis-Tris Plus gels (Invitrogen, NW04125). .. After semidry transfer, the immobilon membranes were blocked with 5% bovine serum albumin in 0.1% Tween-20 in TBS (150 mM NaCl, 50 mM TrisHCl pH 7.5) for 1 h and then incubated overnight at 4 °C in blocking buffer containing primary antibodies recognizing EphA4 (BD Biosciences, 610471; 1:1000; D , , A – C , B and A , an affinity-purified rabbit polyclonal EphA4 antibody generated using a peptide corresponding to the 11 C-terminal amino acids of human and mouse EphA4 ( ) and used at 1 μg/ml in D and A ); EphA3 pY779 (Cell Signaling Technology, 8862; 1:1000 dilution), which also recognizes EphA4 pY779; β-tubulin (Cell Signaling Technology, 2128; 1:25,000 dilution); the FLAG tag (Sigma, F1804; 1:1000 dilution) and an HRP-conjugated antibody recognizing phosphotyrosine (Cell Signaling Technology, 5465; 1:2000). .. The Strep tag was detected by incubating the blots overnight with Strep-Tactin-HRP conjugate (IBA GmbH, 2-1502-001; 1:1000 dilution).

    Blocking Assay:

    Article Title: A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain
    Article Snippet: Lysates, pull-downs and immunoprecipitations were run on Bolt 4-12% Bis-Tris Plus gels (Invitrogen, NW04125). .. After semi-dry transfer, the immobilon membranes were blocked with 5% bovine serum albumin in 0.1% Tween-20 in TBS (150 mM NaCl, 50 mM TrisHCl pH 7.5) for 1 hour and then incubated overnight at 4°C in blocking buffer containing primary antibodies recognizing EphA4 (BD Biosciences, 610471; 1:1,000; Figs. 1D, 2A-C, 4B and 7A), an affinitypurified rabbit polyclonal EphA4 antibody generated using a peptide corresponding the the 11 C- terminal amino acids of human and mouse EphA4 (76) and used at 1 g/ml in Figs. 2D and 4A); EphA3 pY779 (Cell Signaling Technology, 8862; 1:1,000 dilution), which also recognizes EphA4 pY779; β-tubulin (Cell Signaling Technology, 2128; 1:25,000 dilution); the FLAG tag (Sigma, F1804; 1:1,000 dilution) and an HRP conjugated antibody recognizing phosphotyrosine (Cell Signaling Technology, 5465; 1:2,000). .. The Strep tag was detected by incubating the blots overnight with Strep-Tactin-HRP conjugate (IBA GmbH, 2-1502-001; 1:1,000 dilution).

    Article Title: A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain
    Article Snippet: Lysates, pull-downs, and immunoprecipitations were run on Bolt 4 to 12% Bis-Tris Plus gels (Invitrogen, NW04125). .. After semidry transfer, the immobilon membranes were blocked with 5% bovine serum albumin in 0.1% Tween-20 in TBS (150 mM NaCl, 50 mM TrisHCl pH 7.5) for 1 h and then incubated overnight at 4 °C in blocking buffer containing primary antibodies recognizing EphA4 (BD Biosciences, 610471; 1:1000; D , , A – C , B and A , an affinity-purified rabbit polyclonal EphA4 antibody generated using a peptide corresponding to the 11 C-terminal amino acids of human and mouse EphA4 ( ) and used at 1 μg/ml in D and A ); EphA3 pY779 (Cell Signaling Technology, 8862; 1:1000 dilution), which also recognizes EphA4 pY779; β-tubulin (Cell Signaling Technology, 2128; 1:25,000 dilution); the FLAG tag (Sigma, F1804; 1:1000 dilution) and an HRP-conjugated antibody recognizing phosphotyrosine (Cell Signaling Technology, 5465; 1:2000). .. The Strep tag was detected by incubating the blots overnight with Strep-Tactin-HRP conjugate (IBA GmbH, 2-1502-001; 1:1000 dilution).

    Generated:

    Article Title: A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain
    Article Snippet: Lysates, pull-downs and immunoprecipitations were run on Bolt 4-12% Bis-Tris Plus gels (Invitrogen, NW04125). .. After semi-dry transfer, the immobilon membranes were blocked with 5% bovine serum albumin in 0.1% Tween-20 in TBS (150 mM NaCl, 50 mM TrisHCl pH 7.5) for 1 hour and then incubated overnight at 4°C in blocking buffer containing primary antibodies recognizing EphA4 (BD Biosciences, 610471; 1:1,000; Figs. 1D, 2A-C, 4B and 7A), an affinitypurified rabbit polyclonal EphA4 antibody generated using a peptide corresponding the the 11 C- terminal amino acids of human and mouse EphA4 (76) and used at 1 g/ml in Figs. 2D and 4A); EphA3 pY779 (Cell Signaling Technology, 8862; 1:1,000 dilution), which also recognizes EphA4 pY779; β-tubulin (Cell Signaling Technology, 2128; 1:25,000 dilution); the FLAG tag (Sigma, F1804; 1:1,000 dilution) and an HRP conjugated antibody recognizing phosphotyrosine (Cell Signaling Technology, 5465; 1:2,000). .. The Strep tag was detected by incubating the blots overnight with Strep-Tactin-HRP conjugate (IBA GmbH, 2-1502-001; 1:1,000 dilution).

    Article Title: A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain
    Article Snippet: Lysates, pull-downs, and immunoprecipitations were run on Bolt 4 to 12% Bis-Tris Plus gels (Invitrogen, NW04125). .. After semidry transfer, the immobilon membranes were blocked with 5% bovine serum albumin in 0.1% Tween-20 in TBS (150 mM NaCl, 50 mM TrisHCl pH 7.5) for 1 h and then incubated overnight at 4 °C in blocking buffer containing primary antibodies recognizing EphA4 (BD Biosciences, 610471; 1:1000; D , , A – C , B and A , an affinity-purified rabbit polyclonal EphA4 antibody generated using a peptide corresponding to the 11 C-terminal amino acids of human and mouse EphA4 ( ) and used at 1 μg/ml in D and A ); EphA3 pY779 (Cell Signaling Technology, 8862; 1:1000 dilution), which also recognizes EphA4 pY779; β-tubulin (Cell Signaling Technology, 2128; 1:25,000 dilution); the FLAG tag (Sigma, F1804; 1:1000 dilution) and an HRP-conjugated antibody recognizing phosphotyrosine (Cell Signaling Technology, 5465; 1:2000). .. The Strep tag was detected by incubating the blots overnight with Strep-Tactin-HRP conjugate (IBA GmbH, 2-1502-001; 1:1000 dilution).

    FLAG-tag:

    Article Title: A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain
    Article Snippet: Lysates, pull-downs and immunoprecipitations were run on Bolt 4-12% Bis-Tris Plus gels (Invitrogen, NW04125). .. After semi-dry transfer, the immobilon membranes were blocked with 5% bovine serum albumin in 0.1% Tween-20 in TBS (150 mM NaCl, 50 mM TrisHCl pH 7.5) for 1 hour and then incubated overnight at 4°C in blocking buffer containing primary antibodies recognizing EphA4 (BD Biosciences, 610471; 1:1,000; Figs. 1D, 2A-C, 4B and 7A), an affinitypurified rabbit polyclonal EphA4 antibody generated using a peptide corresponding the the 11 C- terminal amino acids of human and mouse EphA4 (76) and used at 1 g/ml in Figs. 2D and 4A); EphA3 pY779 (Cell Signaling Technology, 8862; 1:1,000 dilution), which also recognizes EphA4 pY779; β-tubulin (Cell Signaling Technology, 2128; 1:25,000 dilution); the FLAG tag (Sigma, F1804; 1:1,000 dilution) and an HRP conjugated antibody recognizing phosphotyrosine (Cell Signaling Technology, 5465; 1:2,000). .. The Strep tag was detected by incubating the blots overnight with Strep-Tactin-HRP conjugate (IBA GmbH, 2-1502-001; 1:1,000 dilution).

    Article Title: A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain
    Article Snippet: Lysates, pull-downs, and immunoprecipitations were run on Bolt 4 to 12% Bis-Tris Plus gels (Invitrogen, NW04125). .. After semidry transfer, the immobilon membranes were blocked with 5% bovine serum albumin in 0.1% Tween-20 in TBS (150 mM NaCl, 50 mM TrisHCl pH 7.5) for 1 h and then incubated overnight at 4 °C in blocking buffer containing primary antibodies recognizing EphA4 (BD Biosciences, 610471; 1:1000; D , , A – C , B and A , an affinity-purified rabbit polyclonal EphA4 antibody generated using a peptide corresponding to the 11 C-terminal amino acids of human and mouse EphA4 ( ) and used at 1 μg/ml in D and A ); EphA3 pY779 (Cell Signaling Technology, 8862; 1:1000 dilution), which also recognizes EphA4 pY779; β-tubulin (Cell Signaling Technology, 2128; 1:25,000 dilution); the FLAG tag (Sigma, F1804; 1:1000 dilution) and an HRP-conjugated antibody recognizing phosphotyrosine (Cell Signaling Technology, 5465; 1:2000). .. The Strep tag was detected by incubating the blots overnight with Strep-Tactin-HRP conjugate (IBA GmbH, 2-1502-001; 1:1000 dilution).

    Affinity Purification:

    Article Title: A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain
    Article Snippet: Lysates, pull-downs, and immunoprecipitations were run on Bolt 4 to 12% Bis-Tris Plus gels (Invitrogen, NW04125). .. After semidry transfer, the immobilon membranes were blocked with 5% bovine serum albumin in 0.1% Tween-20 in TBS (150 mM NaCl, 50 mM TrisHCl pH 7.5) for 1 h and then incubated overnight at 4 °C in blocking buffer containing primary antibodies recognizing EphA4 (BD Biosciences, 610471; 1:1000; D , , A – C , B and A , an affinity-purified rabbit polyclonal EphA4 antibody generated using a peptide corresponding to the 11 C-terminal amino acids of human and mouse EphA4 ( ) and used at 1 μg/ml in D and A ); EphA3 pY779 (Cell Signaling Technology, 8862; 1:1000 dilution), which also recognizes EphA4 pY779; β-tubulin (Cell Signaling Technology, 2128; 1:25,000 dilution); the FLAG tag (Sigma, F1804; 1:1000 dilution) and an HRP-conjugated antibody recognizing phosphotyrosine (Cell Signaling Technology, 5465; 1:2000). .. The Strep tag was detected by incubating the blots overnight with Strep-Tactin-HRP conjugate (IBA GmbH, 2-1502-001; 1:1000 dilution).



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